{"id":2124,"date":"2023-02-01T13:41:39","date_gmt":"2023-02-01T11:41:39","guid":{"rendered":"http:\/\/150.140.204.29\/ServiceDB\/?page_id=2124"},"modified":"2023-02-01T16:08:07","modified_gmt":"2023-02-01T14:08:07","slug":"frequently-asked-questions-instructions","status":"publish","type":"page","link":"http:\/\/150.140.204.29\/ServiceDB\/?page_id=2124","title":{"rendered":"FAQs"},"content":{"rendered":"\t\t
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Frequently Asked Questions - Instructions<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Collecting, Preserving and Sending Samples<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\tSampling instructions<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t
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  1. The sample must be representative. If it is a large tissue or a whole fish, the point where DNA isolation will be done should be indicated.<\/li>\n
  2. If several tissue pieces are sent in the same tube, the selection of the sample by BioDetect for DNA isolation will be random. Generally, this sample shipping option is not recommended.<\/li>\n
  3. The quantity of sample should be sufficient for the tests requested. In the case where only DNA isolation is required, a small piece of tissue is sufficient.<\/li>\n
  4. Necessary handling during sampling requires sterile tools (forceps, spatulas, spoons, packages) and the sampler’s hands should be covered with sterile gloves.<\/li>\n
  5. The information (Unique sample code \/ point and time of sampling) is noted on a label, which is stuck on the container. It is recommended to avoid sending samples without any code. Also, it is recommended to avoid simple codes like 1, 2, 3\u2026 or A, B, C… for easier later identification of the results.<\/li>\n<\/ol>\n

    Below is a summary Table<\/b> with instructions for sample preparation.<\/p>\n\n\n\n\n\n\n\n
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    Analysis<\/strong><\/p>\n<\/td>\n

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    Sample<\/strong><\/p>\n<\/td>\n

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    Quantity<\/strong><\/p>\n<\/td>\n

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    Preservation<\/strong><\/p>\n<\/td>\n

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    Sampling Container<\/strong><\/p>\n<\/td>\n<\/tr>\n

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    PCR<\/strong><\/p>\n<\/td>\n

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    Tissues<\/strong> (spleen, kidney etc)<\/p>\n<\/td>\n

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    Whole organ or representative tissue pieces >100mg<\/strong><\/p>\n<\/td>\n

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    RNAlater<\/strong><\/p>\n

    (1 day at 37\u00b0C, 1 week at 25\u00b0C, 1 month or more at 4\u00b0C)<\/p>\n<\/td>\n

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    Sterile tube type Eppendorf (1.5 ml) or type Universal (20 ml) or type Falcon (50 ml)<\/p>\n<\/td>\n<\/tr>\n

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    PCR<\/strong><\/p>\n<\/td>\n

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    Eggs<\/strong><\/p>\n<\/td>\n

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    >2<\/strong> ml<\/strong><\/p>\n<\/td>\n

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    As is in the sample water<\/strong><\/p>\n

    (Keep at 4\u00b0C, do not freeze, if shipped the same day)<\/strong><\/p>\n<\/td>\n

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    Sterile tube type Eppendorf (1.5 ml) or type Universal (20 ml) or type Falcon (50 ml)<\/p>\n<\/td>\n<\/tr>\n

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    Staining<\/strong><\/p>\n<\/td>\n

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    Tissues<\/strong> (spleen, kidney etc)<\/p>\n<\/td>\n

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    Whole organ<\/strong><\/p>\n<\/td>\n

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    Avoid freezing, fast shipping on ice in sterile buffer or without any preservation fluid<\/strong><\/p>\n<\/td>\n

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    Sterile tube type Eppendorf (1.5 ml) or type Universal (20 ml) or type Falcon (50 ml)<\/p>\n<\/td>\n<\/tr>\n

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    Culturing<\/strong><\/p>\n<\/td>\n

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    Tissues<\/strong> (spleen, kidney etc)<\/p>\n<\/td>\n

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    Whole organ<\/strong><\/p>\n<\/td>\n

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    Avoid freezing, fast shipping on ice in sterile buffer or without any preservation fluid <\/strong><\/p>\n<\/td>\n

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    Sterile tube type Eppendorf (1.5 ml) or type Universal (20 ml) or type Falcon (50 ml)<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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    \n\t\t\t\t\t\t\t\t\t\t\t\tArranging shipment<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t
    1. Please note that shipping arrangements are made exclusively by our customers and the full cost of shipping\/sending of samples is met by our customers and not BioDetect.<\/li>
    2. We recommend that samples are sent to us via courier or recorded delivery to ensure full track and trace of your consignment.<\/li>
    3. Please send us a quick email to let us know that the samples are on the way once this is arranged and provide us with the tracking number if that is available.<\/li>
    4. Our full postal address:
      BioDetect
      <\/strong>Department of Sustainable Agriculture
      <\/strong>University of Patras
      <\/strong>2 Seferi str., Agrinio
      <\/strong>30100, Greece<\/strong>Consideration: George Tsiamis (+30 6976 685012) \/ Panayota Stathopoulou (+30 6977 479169)<\/strong><\/li><\/ol>
      1. Our email<\/strong> address: biodetect@upatras.gr<\/strong><\/a><\/li><\/ol><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t
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        \n\t\t\t\t\t\t\t\t\t\t\t\tResponse time<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

        Response times vary depending on the type of analysis. Usual response times for the most common analyses include:<\/p>