{"id":7121,"date":"2022-08-03T14:13:22","date_gmt":"2022-08-03T11:13:22","guid":{"rendered":"http:\/\/150.140.204.29\/?p=7121"},"modified":"2022-08-03T14:13:52","modified_gmt":"2022-08-03T11:13:52","slug":"detection-and-characterization-of-wolbachia-infections-in-laboratory-and-natural-populations-of-different-species-of-tsetse-flies-genus-glossina-2","status":"publish","type":"post","link":"http:\/\/150.140.204.29\/?p=7121&lang=el","title":{"rendered":"Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina)"},"content":{"rendered":"\t\t
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Background<\/h3>

Wolbachia<\/i> is a genus of endosymbiotic \u03b1-Proteobacteria infecting a wide range of arthropods and filarial nematodes. Wolbachia<\/i> is able to induce reproductive abnormalities such as cytoplasmic incompatibility (CI), thelytokous parthenogenesis, feminization and male killing, thus affecting biology, ecology and evolution of its hosts. The bacterial group has prompted research regarding its potential for the control of agricultural and medical disease vectors, including Glossina<\/i> spp., which transmits African trypanosomes, the causative agents of sleeping sickness in humans and nagana in animals.<\/p>

Results<\/h3>

In the present study, we employed a Wolbachia<\/i> specific 16S rRNA<\/i> PCR assay to investigate the presence of Wolbachia<\/i> in six different laboratory stocks as well as in natural populations of nine different Glossina<\/i> species originating from 10 African countries. Wolbachia<\/i> was prevalent in Glossina morsitans morsitans<\/i>, G. morsitans centralis<\/i> and G. austeni<\/i> populations. It was also detected in G. brevipalpis<\/i>, and, for the first time, in G. pallidipes<\/i> and G. palpalis gambiensis<\/i>. On the other hand, Wolbachia<\/i> was not found in G. p. palpalis<\/i>, G. fuscipes fuscipes<\/i> and G. tachinoides<\/i>. Wolbachia<\/i> infections of different laboratory and natural populations of Glossina<\/i> species were characterized using 16S rRNA<\/i>, the wsp<\/i> (Wolbachia Surface Protein) gene and MLST (Multi Locus Sequence Typing) gene markers. This analysis led to the detection of horizontal gene transfer events, in which Wobachia<\/i> genes were inserted into the tsetse flies fly nuclear genome.<\/p>

Conclusions<\/h3>

Wolbachia<\/i> infections were detected in both laboratory and natural populations of several different Glossina<\/i> species. The characterization of these Wolbachia<\/i> strains promises to lead to a deeper insight in tsetse flies-Wolbachia<\/i> interactions, which is essential for the development and use of Wolbachia<\/i>-based biological control methods.<\/p><\/div><\/div>

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https:\/\/doi.org\/10.1186\/1471-2180-12-S1-S3<\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

\u03a0\u03b5\u03c1\u03af\u03bb\u03b7\u03c8\u03b7 Background Wolbachia is a genus of endosymbiotic \u03b1-Proteobacteria infecting a wide range of arthropods and filarial nematodes. Wolbachia is able to induce reproductive abnormalities such as cytoplasmic incompatibility (CI), thelytokous parthenogenesis, feminization and male killing, thus affecting biology, ecology and evolution of its hosts. The bacterial group has prompted research regarding its potential for […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","ocean_post_oembed":"","ocean_post_self_hosted_media":"","ocean_post_video_embed":"","ocean_link_format":"","ocean_link_format_target":"self","ocean_quote_format":"","ocean_quote_format_link":"post","ocean_gallery_link_images":"off","ocean_gallery_id":[],"footnotes":""},"categories":[24],"tags":[],"class_list":["post-7121","post","type-post","status-publish","format-standard","hentry","category-publications-el","entry"],"yoast_head":"\nDetection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina) – Laboratory of Systems Microbiology and Applied Genomics<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/150.140.204.29\/?p=7121&lang=el\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina) – Laboratory of Systems Microbiology and Applied Genomics\" \/>\n<meta property=\"og:description\" content=\"\u03a0\u03b5\u03c1\u03af\u03bb\u03b7\u03c8\u03b7 Background Wolbachia is a genus of endosymbiotic \u03b1-Proteobacteria infecting a wide range of arthropods and filarial nematodes. Wolbachia is able to induce reproductive abnormalities such as cytoplasmic incompatibility (CI), thelytokous parthenogenesis, feminization and male killing, thus affecting biology, ecology and evolution of its hosts. 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